Tissue-specific expression of glutathione peroxidase gene in guinea pigs

Glutathione peroxidase (GSH-Px) in guinea pigs was used Northern blot analysis and nuclei run on assay to measure gene expression of GSH-Px activity in major tissues such as liver and kidney in guinea pigs. GSH-Px is a selenocysteine containing enzyme that is important in protecting animals form oxidative injury. Guinea pigs were used on this experiment, due to their very low GSH-Px activity in major tissues. This study present the gene expression of GSH-Px is responsible for the tissues specific regulation of GSH-Px activity in guinea pigs. The result showed that GSH-Px gene in guinea pigs is inactive in any tissues, it has activity transcribed in erythroblasts or erythroid cells on the compare study with mice this may due to the necessity of an antioxidant in the erythrocytes which convey oxygen and susceptible to oxidative stress.

Winnie Yum