fluorescence in situ hybridization

FISH stands for fluorescence in situ hybridization. It is a cytogenetic technique which is commonly used to probe the presence or absence of a particular DNA sequences on chromosomes. Fluorescent probes only bind to those parts of the chromosome with which they show a high degree of sequence similarity. In this paper, multiple expression domains for SpBrn1/2/4 have been discovered by fluorescent in situ hybridization. Endodermal expression of this gene can be detected in the foregut. SpBrn1/2/4 is also expressed in two distinct ectodermal domains: throughout the stomodeal ectoderm, and within cells scattered throughout the ciliated band. In addition, with this cytogenetic technique, scientists have discovered a unique ectodermal cell type that co-expresses the ParaHox gene SpLox.

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http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W8W-4VPV5GT-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1043198949&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=be121d4546e01497a6436e71b4659109

Qiushi Chen
41560637