Nanopore Sequencing
“Faster- Cheaper -Easier -More Accurate”
Have you ever thought of protein nanopores, able to read what is there in your DNA?? Surprised, but group of scientist have discovered a novel way of de novo DNA sequencing using protein nanopores. This could be the break through in next generation sequencing approach and could sequence a mammalian genome in approximately not more than a single day in just 1000 dollars. What could be more amazing than this when one could easily find out what is stored in ones genome, in a cost-effective way and much faster than one could imagine. Nanopore sequencing has the potential to sequence nucleotides label free and without the need of amplification. It makes use of a biological nanopore, Staphylococcal alpha-haemolysin protein pore.It is when across the protein pore channel the DNA strand is passed,every nucleotide creates modulation in the ionic current and thus can be detected, as each of the nucelotide base (A,T,G,C) show acharacteristic decrease in current amplitude.It also can distinguish methylated cytosine from the four standard DNA bases, showing its potential in studying epigenetic modifications. At the same time it may also open the possibility for the high-resolution analysis of chromosomal structure variation, and long-range haplotype mapping. This third generation sequencing is currently being developed by Oxford Nanopore Technologies.
To get fascinated more, click here http://www.nature.com/nnano/journal/v4/n4/abs/nnano.2009.12.html
References:
1. James Clarke, Hai-Chen Wu, Lakmal Jayasinghe, Alpesh Patel1, Stuart Reid & Hagan Bayley. Continuous base identification for single-molecule nanopore DNA sequencing. Nature Nanotechnology 4, 265 - 270 (2009)
2. Daniel Branton et al. The potential and challenges of nanopore sequencing. Nature Biotechnology 26, 1146 - 1153 (2008)
Samikshya Biswal
41568400
Have you ever thought of protein nanopores, able to read what is there in your DNA?? Surprised, but group of scientist have discovered a novel way of de novo DNA sequencing using protein nanopores. This could be the break through in next generation sequencing approach and could sequence a mammalian genome in approximately not more than a single day in just 1000 dollars. What could be more amazing than this when one could easily find out what is stored in ones genome, in a cost-effective way and much faster than one could imagine. Nanopore sequencing has the potential to sequence nucleotides label free and without the need of amplification. It makes use of a biological nanopore, Staphylococcal alpha-haemolysin protein pore.It is when across the protein pore channel the DNA strand is passed,every nucleotide creates modulation in the ionic current and thus can be detected, as each of the nucelotide base (A,T,G,C) show acharacteristic decrease in current amplitude.It also can distinguish methylated cytosine from the four standard DNA bases, showing its potential in studying epigenetic modifications. At the same time it may also open the possibility for the high-resolution analysis of chromosomal structure variation, and long-range haplotype mapping. This third generation sequencing is currently being developed by Oxford Nanopore Technologies.
To get fascinated more, click here http://www.nature.com/nnano/journal/v4/n4/abs/nnano.2009.12.html
References:
1. James Clarke, Hai-Chen Wu, Lakmal Jayasinghe, Alpesh Patel1, Stuart Reid & Hagan Bayley. Continuous base identification for single-molecule nanopore DNA sequencing. Nature Nanotechnology 4, 265 - 270 (2009)
2. Daniel Branton et al. The potential and challenges of nanopore sequencing. Nature Biotechnology 26, 1146 - 1153 (2008)
Samikshya Biswal
41568400
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