ChIP-seq technique
ChIP-seq TECHNIQUE
ChIP-Seq is an emerging technique of chromatin immunoprecipitation for the research on gene regulation and epigenetic mechanism. Comparing with conventional ChIP-chip methods, the application of next generation sequencing ChIP-seq that is available for measurement of gene expression through epigenome profiling of DNA- protein interaction, histone modification and nucleosome gives higher resolution with less noise and greater coverage. One current research on gene regulation has defined that tissue-specific activity of enhancers can be accurately predicted by using ChIP-seq.
References
Park, P.J. (2009). ChIP–seq: advantages and challenges of a maturing technology Nature reviews genetics, 10: 669-680.
Visel, A; Matthew, J.; Blow, M.J.; Li Z.; Zhang, T.; Akiyama, J.A.; Holt, A.; Plajzer-Frick I, Shoukry, M.; Wright, C.; Chen, F. et al (2009). Nature, 457: 854-858
Dinh Hoang Lan Chi
Student No: 41614987
ChIP-Seq is an emerging technique of chromatin immunoprecipitation for the research on gene regulation and epigenetic mechanism. Comparing with conventional ChIP-chip methods, the application of next generation sequencing ChIP-seq that is available for measurement of gene expression through epigenome profiling of DNA- protein interaction, histone modification and nucleosome gives higher resolution with less noise and greater coverage. One current research on gene regulation has defined that tissue-specific activity of enhancers can be accurately predicted by using ChIP-seq.
References
Park, P.J. (2009). ChIP–seq: advantages and challenges of a maturing technology Nature reviews genetics, 10: 669-680.
Visel, A; Matthew, J.; Blow, M.J.; Li Z.; Zhang, T.; Akiyama, J.A.; Holt, A.; Plajzer-Frick I, Shoukry, M.; Wright, C.; Chen, F. et al (2009). Nature, 457: 854-858
Dinh Hoang Lan Chi
Student No: 41614987
Labels: ChIP-seq
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