Promoter swapping, a powerful technique

The ability to control gene expression for many applications, including biomass production using metabolic pathways and functional analysis of cellular processes, has become feasible due to the development of a powerful technique called promoter swapping. This technique which is one type of recombineering involves in the replacement of promoter sequences with engineered promoters using homologous recombination catalysed by phage-derived recombination proteins to alter gene expression. Recombineering was applied firstly in E.coli, and then extended to other organisms including Yersinia, Vibrio, Streptomyces, Mycobacterium, and Pseudomonas.

For marvellous ability of this technique, refer to:

McCleary , William R, 2009. Application of promoter swapping techniques to control expression of chromosomal genes. Appl Microbiol Biotechnol, 84, 641-648.
http://www.springerlink.com/content/63kl5837t483527q/fulltext.html

My Ngoc Nghiem
ID: 42136860