Polony Multiplex Analysis of Gene Expression (PMAGE)

Several methods have been developed to define the complete repertoire of RNA transcripts including microarrays and serial analysis of gene expression. However many of these fail in detecting rare transcripts or mRNA at low expression and hold with them multiple limitations. Polony multiplex analysis of gene expression (PMAGE) is developed to detect mRNA as rare as one transcript per three cells. One sequence tag from each mRNA molecule is amplified onto a separate 1-micrometer bead, denoted as polymerase colony or polony. Approximately 5 million polonies are arrayed in a flow cell for parallel sequencing. One of the applications of this method is identifying early transcriptional changes that preceded pathological manifestation of hypertrophic cardiomyopathy in mice carrying a disease-causing mutation.

Posted by Uyen Tran
ID: 41987494