Interesting New Findings of DNA Methylation in Human Colon Cancer
Nurrahmi Dewi Fajarningsih
(41916243)
Previous studies of DNA methylation in cancer are mainly focus on CpG methylation and promotor hypermethylation. However, a complete genome-scale analysis of DNA methylation (DNAm) in order to get a comprehensive understanding about variation of normal and cancer cells have not been carried out. To address that, Irizary et al (2009) conducted a comprehensive high-throughput array based relative methylation (CHARM) genome-wide analysis and found some interesting result. The first interesting finding is that most of tissue specific DNAm are not occurred at CpG islands but at ‘CpG island shores’ (located within 2 kb of islands). Second, they also identified that differential methylation regions changes (C-DMRs) in colon cancer are similarly divided between hypomethylation (44%) and hypermethylation (56%). Third, C-DMRs and tissues differential methylation regions (T-DMRs) are both located at CpG island shores and both are 45-65% overlapped. As a result, spesific DNA methylation changes of cancer involving the same DMRs of normal tissue (involve the same site).
(41916243)
Previous studies of DNA methylation in cancer are mainly focus on CpG methylation and promotor hypermethylation. However, a complete genome-scale analysis of DNA methylation (DNAm) in order to get a comprehensive understanding about variation of normal and cancer cells have not been carried out. To address that, Irizary et al (2009) conducted a comprehensive high-throughput array based relative methylation (CHARM) genome-wide analysis and found some interesting result. The first interesting finding is that most of tissue specific DNAm are not occurred at CpG islands but at ‘CpG island shores’ (located within 2 kb of islands). Second, they also identified that differential methylation regions changes (C-DMRs) in colon cancer are similarly divided between hypomethylation (44%) and hypermethylation (56%). Third, C-DMRs and tissues differential methylation regions (T-DMRs) are both located at CpG island shores and both are 45-65% overlapped. As a result, spesific DNA methylation changes of cancer involving the same DMRs of normal tissue (involve the same site).
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