Regulation of pregnane X receptor gene by protein arginine methyltransferase 1: new information for drug designs.
Posted by: Thanh Nga Pham
Student number: 41912124
Imagine when you take a drug, how can it be transformed in the body. Infact, there are many xenobiotics, which are foreign compounds, including drugs or poisons, in organism’s body. Understanding the regulation of xenobiotic metabolism, which is a process modifying xenobiotics, can support the development of drugs and therapies. Xenobiotic metabolism is demonstrated to be regulated by a ligand-dependent trascription factor called pregnane X receptor (PXR). The trascription of PXR depends on protein arginine methyltransferase 1 (PRMT1). PRMT1 acts by methylating histone and non-histone proteins and thus being known to play an significant role in regulating expression of many genes. This research aims to discover the role of PRMT1 in the regulation of PXR gene.
The result demonstrated that PRMT1 had close histone methyltransferase (HMT) associated with PXR, and therefore giving evidence that PRMT1 is needed for PXR transcription. Furthermore, this interaction is in a specific ligand-dependent manner. In this interaction, PRMT1 showed inhibiting influence on the PXR transcriptional activity. This inhibition may indicate that the expression of PXR-regulated gene needs the present of PRMT1. However, PXR also showed significant effect on regulation of PRMT1 subcellular localization. PRMT1 was localized in cytoplasm in absence of PXR, while it was localized in nucleus in present of PXR.
To sum up, this reseach proved reciprocal association between PXR and PRMT1, which may be important for the regulation of both PXR and PRMT1 gene expression.
For more information, follow the link to read the full article.
Student number: 41912124
Imagine when you take a drug, how can it be transformed in the body. Infact, there are many xenobiotics, which are foreign compounds, including drugs or poisons, in organism’s body. Understanding the regulation of xenobiotic metabolism, which is a process modifying xenobiotics, can support the development of drugs and therapies. Xenobiotic metabolism is demonstrated to be regulated by a ligand-dependent trascription factor called pregnane X receptor (PXR). The trascription of PXR depends on protein arginine methyltransferase 1 (PRMT1). PRMT1 acts by methylating histone and non-histone proteins and thus being known to play an significant role in regulating expression of many genes. This research aims to discover the role of PRMT1 in the regulation of PXR gene.
The result demonstrated that PRMT1 had close histone methyltransferase (HMT) associated with PXR, and therefore giving evidence that PRMT1 is needed for PXR transcription. Furthermore, this interaction is in a specific ligand-dependent manner. In this interaction, PRMT1 showed inhibiting influence on the PXR transcriptional activity. This inhibition may indicate that the expression of PXR-regulated gene needs the present of PRMT1. However, PXR also showed significant effect on regulation of PRMT1 subcellular localization. PRMT1 was localized in cytoplasm in absence of PXR, while it was localized in nucleus in present of PXR.
To sum up, this reseach proved reciprocal association between PXR and PRMT1, which may be important for the regulation of both PXR and PRMT1 gene expression.
For more information, follow the link to read the full article.
0 Comments:
Post a Comment
<< Home