New discovery about liver cancer cells: SP1 is an essential aspect for activation and chromatin availability of CD151 promoter

Liver cancer is one of the most common malignant neoplastic diseases today. CD151 gene was known as an influential factor of this cancer’s metastasis. It means that ability to detect liver cancer in diagnostics will be more difficult if the level of CD151 is high. Besides, specific protein 1(SP1) plays activating aspect role in transcription.

By using chromatin accessibility analysis in real-time PCR, Wang and colleagues discovered that there was an open chromatin structure lied in the transcription starting site region of CD151 gene. Before being transferred into HepG2 and Hep3B cells, some fragments in the 5’end of CD151 gene were cut and then it was bound with luciferase reporter gene. They found that SP1 was linked with promoter’s core by manipulating electrophoretic mobility shift assay as well as chromatin immunoprecipitation assay. Their research’s result also showed that the CD151 promoter will completely lost its activity when SP1 was removed.

NGUYEN Thi Thu Hang
41882601

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